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Primer and target sequences used in this study.
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Primer and target sequences used in this study.

Journal: International Journal of Molecular Medicine

Article Title: The role of lncRNA MALAT1 in the regulation of hepatocyte proliferation during liver regeneration

doi: 10.3892/ijmm.2017.2854

Figure Lengend Snippet: Primer and target sequences used in this study.

Article Snippet: Cyclin E1 (11554-1-AP) , 1:1,000 , , , Rabbit polyclonal , Proteintech.

Techniques:

Information regarding the antibodies used in this study.

Journal: International Journal of Molecular Medicine

Article Title: The role of lncRNA MALAT1 in the regulation of hepatocyte proliferation during liver regeneration

doi: 10.3892/ijmm.2017.2854

Figure Lengend Snippet: Information regarding the antibodies used in this study.

Article Snippet: Cyclin E1 (11554-1-AP) , 1:1,000 , , , Rabbit polyclonal , Proteintech.

Techniques: Immunohistochemistry-IF

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) accelerates the hepatocyte cell cycle and inhibits apoptosis in vitro . (A) The expression of MALAT1 in BNL CL.2 cells and NCTC 1469 cells was determined by RT-qPCR. The level of MALAT1 in NCTC 1469 cells was set to a value of 1. (B) RT-qPCR analysis of MALAT1 expression after MALAT1 was knocked down by siRNA in BNL CL.2 cells. MALAT1 expression was determined by RT-qPCR after MALAT1 was overexpressed in NCTC 1469 cells. (C) Cell proliferation was assessed by CCK-8 assay. (D) Cell proliferation was assessed using 5-ethynyl-2 ′ -deoxyuridine (EdU) immunofluorescence staining. (E) FACS analysis showing that the number of cells in the G1/G0 phase increased significantly in MALAT1-silenced BNL CL.2 cells and that the number of cells in the S phase increased in MALAT1-overexpressing NCTC 1469 cells. (F) FACS analysis showing a significant increase in the number of apoptotic cells when MALAT1 was silenced in BNL CL.2 cells and a decrease in the number of apoptotic NCTC 1469 cells following transfection with pcDNA3.1-MALAT1. The percentage of apoptotic cells is also shown. (G) Western blot analysis of the expression of cyclin B1 and E1 in MALAT1-silenced BNL CL.2 cells at different time points during the cell cycle. The data are presented as the means ± SD of 3 independent experiments. * p<0.05, ** p<0.01 vs the control or the 0 h time point.

Journal: International Journal of Molecular Medicine

Article Title: The role of lncRNA MALAT1 in the regulation of hepatocyte proliferation during liver regeneration

doi: 10.3892/ijmm.2017.2854

Figure Lengend Snippet: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) accelerates the hepatocyte cell cycle and inhibits apoptosis in vitro . (A) The expression of MALAT1 in BNL CL.2 cells and NCTC 1469 cells was determined by RT-qPCR. The level of MALAT1 in NCTC 1469 cells was set to a value of 1. (B) RT-qPCR analysis of MALAT1 expression after MALAT1 was knocked down by siRNA in BNL CL.2 cells. MALAT1 expression was determined by RT-qPCR after MALAT1 was overexpressed in NCTC 1469 cells. (C) Cell proliferation was assessed by CCK-8 assay. (D) Cell proliferation was assessed using 5-ethynyl-2 ′ -deoxyuridine (EdU) immunofluorescence staining. (E) FACS analysis showing that the number of cells in the G1/G0 phase increased significantly in MALAT1-silenced BNL CL.2 cells and that the number of cells in the S phase increased in MALAT1-overexpressing NCTC 1469 cells. (F) FACS analysis showing a significant increase in the number of apoptotic cells when MALAT1 was silenced in BNL CL.2 cells and a decrease in the number of apoptotic NCTC 1469 cells following transfection with pcDNA3.1-MALAT1. The percentage of apoptotic cells is also shown. (G) Western blot analysis of the expression of cyclin B1 and E1 in MALAT1-silenced BNL CL.2 cells at different time points during the cell cycle. The data are presented as the means ± SD of 3 independent experiments. * p<0.05, ** p<0.01 vs the control or the 0 h time point.

Article Snippet: Cyclin E1 (11554-1-AP) , 1:1,000 , , , Rabbit polyclonal , Proteintech.

Techniques: In Vitro, Expressing, Quantitative RT-PCR, CCK-8 Assay, Immunofluorescence, Staining, Transfection, Western Blot, Control

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) activates the Wnt/β-catenin pathway by suppressing Axin1 and APC. (A) RT-qPCR analysis revealing the levels of β-catenin and cyclin D1 in MALAT1-silenced BNL CL.2 cells and MALAT1-overexpressing NCTC 1469 cells. (B) Changes in the expression of the β-catenin destruction complex (Axin1, APC and GSK-3β) after the silencing or the overexpression of MALAT1 were detected by RT-qPCR. (C) Changes in the expression of the β-catenin destruction complex (Axin1, APC and GSK-3β) and downstream target genes (cyclin D1 and c-Myc) after the silencing or overexpression of MALAT1 were detected by western blot analysis. The value below each band indicates the fold change in the protein level. (D) Changes in the expression and distribution of β-catenin in the cytoplasm and nucleus were observed by immunofluorescence (IF) staining after the silencing or overexpression of MALAT1. Yellow arrows indicate the translocation of β-catenin (scale bars, 20 μ m). (E) The levels of nuclear, cytoplasmic and total β-catenin protein after the silencing or overexpression of MALAT1 are shown. The data are presented as the mean ± SD of 3 independent experiments. * p<0.05, ** p<0.01 vs. the respective control.

Journal: International Journal of Molecular Medicine

Article Title: The role of lncRNA MALAT1 in the regulation of hepatocyte proliferation during liver regeneration

doi: 10.3892/ijmm.2017.2854

Figure Lengend Snippet: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) activates the Wnt/β-catenin pathway by suppressing Axin1 and APC. (A) RT-qPCR analysis revealing the levels of β-catenin and cyclin D1 in MALAT1-silenced BNL CL.2 cells and MALAT1-overexpressing NCTC 1469 cells. (B) Changes in the expression of the β-catenin destruction complex (Axin1, APC and GSK-3β) after the silencing or the overexpression of MALAT1 were detected by RT-qPCR. (C) Changes in the expression of the β-catenin destruction complex (Axin1, APC and GSK-3β) and downstream target genes (cyclin D1 and c-Myc) after the silencing or overexpression of MALAT1 were detected by western blot analysis. The value below each band indicates the fold change in the protein level. (D) Changes in the expression and distribution of β-catenin in the cytoplasm and nucleus were observed by immunofluorescence (IF) staining after the silencing or overexpression of MALAT1. Yellow arrows indicate the translocation of β-catenin (scale bars, 20 μ m). (E) The levels of nuclear, cytoplasmic and total β-catenin protein after the silencing or overexpression of MALAT1 are shown. The data are presented as the mean ± SD of 3 independent experiments. * p<0.05, ** p<0.01 vs. the respective control.

Article Snippet: Cyclin E1 (11554-1-AP) , 1:1,000 , , , Rabbit polyclonal , Proteintech.

Techniques: Quantitative RT-PCR, Expressing, Over Expression, Western Blot, Immunofluorescence, Staining, Translocation Assay, Control